Columbia University School of Medicine 1 article published in JoVE Developmental Biology Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane Hui Cai1, Jie Gong1, Lucian V. Del Priore1, Tongalp H. Tezel2, Mark A. Fields1 1Department of Ophthalmology and Visual Science, Yale School of Medicine, 2Edward S. Harkness Eye Institute, Columbia University School of Medicine The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.