Ohio State University Comprehensive Cancer Center 2 articles published in JoVE Medicine Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography Qiang Zeng1, Doug A. Gouchoe2,3, Mahboubeh Nabavinia4, Yong Gyu Lee2, Xi Wang2, Terri A. Shaffer5, Mitchel R. Stacy4,5,6, Blake R. Peterson7,8, Bryan A. Whitson2,9, Christopher Breuer1,4, Sylvester M. Black1,10 1Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, 5Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, 6Interdisciplinary Biophysics Graduate Program, The Ohio State University, 7Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, 8The Ohio State University Comprehensive Cancer Center, 9Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, 10Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans. Developmental Biology Analysis of Hematopoietic Stem Progenitor Cell Metabolism Giorgia Scapin1,2,3, Marie C. Goulard1,2,3, Priyanka R. Dharampuriya1,2,3, Jennifer L. Cillis1,2,3, Dhvanit I. Shah1,2,3 1 Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.