USDA-ARS Pollinating Insect Research Unit 1 article published in JoVE Biology A 3D Printed Pollen Trap for Bumble Bee (Bombus) Hive Entrances Houston J. Judd1,2, Craig Huntzinger2, Ricardo Ramirez1, James P. Strange2,3 1Department of Biology, Utah State University, 2USDA-ARS Pollinating Insect Research Unit, 3Department of Entomology, The Ohio State University We present a non-lethal and automated mechanism to collect pollen from bumble bee (Bombus) workers returning to a hive. Instructions for producing, preparing, installing and using the devices are included. By using 3D-printed objects, modification to the design was timely, efficient and allowed for quick turnaround for testing.