Hunter Holmes McGuire VAMC 2 articles published in JoVE Behavior Paradigms of Lower Extremity Electrical Stimulation Training After Spinal Cord Injury Ashraf S. Gorgey1,2, Refka E. Khalil1, Robert M. Lester1, Gary A. Dudley3, David R. Gater4 1Spinal Cord Injury and Disorders Service, Hunter Holmes McGuire VAMC, 2Department of Physical Medicine and Rehabilitation, Virginia Commonwealth University, 3Deceased, Department of Kinesiology, The University of Georgia, 4Department of Physical Medicine and Rehabilitation, Penn State Milton S. Hershey Medical Center Spinal cord injury is a traumatic medical condition that may result in elevated risks of chronic secondary metabolic disorders. Here, we presented a protocol using surface neuromuscular electrical stimulation-resistance training in conjunction with functional electrical stimulation-lower extremities cycling as a strategy to ameliorate several of these medical problems. Biology Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein Mulugeta Seneshaw1, Faridoddin Mirshahi1, Hae-Ki Min1, Amon Asgharpour1, Shervin Mirshahi1, Kalyani Daita1, Sherry Boyett1, Prasanna K. Santhekadur2, Michael Fuchs1, Arun J. Sanyal1 1Division of Gastroenterology, Hepatology and Nutrition, Virginia Commonwealth University, 2McGuire Veterans Affairs Medical Center, Research BC-101 MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.