Sweet Briar College 1 article published in JoVE Biology Rearing the Cabbage White Butterfly (Pieris rapae) in Controlled Conditions: A Case Study with Heavy Metal Tolerance Emilie C. Snell-Rood1, Megan E. Kobiela1,2 1Deptartment of Ecology, Evolution and Behavior, University of Minnesota, 2Biology, Sweet Briar College This paper presents a detailed protocol for rearing the cabbage white butterfly in controlled lab conditions with an artificial diet, which allows precise manipulations of early-life nutrition and toxin exposure. The representative results show how heavy metal toxicity can be assayed with this protocol.