University of Vermont Larner College of Medicine 1 article published in JoVE Neuroscience Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study Amanda C. Rosehart1, Abbie C. Johnson2, Fabrice Dabertrand1,3 1Department of Anesthesiology, University of Colorado Anschutz Medical Campus, 2Department of Neurological Sciences, University of Vermont Larner College of Medicine, 3Department of Pharmacology, University of Colorado Anschutz Medical Campus The present manuscript details how to isolate hippocampal arterioles and capillaries from the mouse brain and how to pressurize them for pressure myography, immunofluorescence, biochemistry, and molecular studies.