SCIREQ Scientific Respiratory Equipment Inc. 3 articles published in JoVE Chemistry Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies Alexandra Noël1, Christina M. Verret1, Farhana Hasan2, Slawomir Lomnicki2, John Morse3, Annette Robichaud3, Arthur L. Penn1 1Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 2Department of Environmental Sciences, College of the Coast & Environment, Louisiana State University, 3SCIREQ Scientific Respiratory Equipment Inc. Electronic cigarette (e-cig) users are increasing worldwide. Little, however, is known about the health effects induced by inhaled e-cig aerosols. This article describes an e-cig aerosol generation technique suitable for animal exposures and subsequent toxicological studies. Such protocols are required to establish experimentally reproducible and standardized e-cig exposure systems. Medicine Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography Samuel Mailhot-Larouche1, Louis Deschênes1, Katherine Lortie1, Morgan Gazzola1, David Marsolais1, David Brunet2, Annette Robichaud2, Ynuk Bossé1 1Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, 2SCIREQ Scientific Respiratory Equipment Inc. The objective of the present article is to provide a detailed description of the recommended procedures to evaluate respiratory function in conscious mice by double-chamber plethysmography. Biology Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique Toby K. McGovern*1, Annette Robichaud*2, Liah Fereydoonzad2, Thomas F. Schuessler2, James G. Martin1 1Meakins-Christie Laboratories, Department of Medicine, McGill University, 2SCIREQ Scientific Respiratory Equipment Inc. The present protocol provides a detailed step-by-step description of the procedures required to execute measurements of respiratory system mechanics as well as the assessment of airway responsiveness to inhaled methacholine in mice using the forced oscillation technique (flexiVent; SCIREQ Inc, Montreal, Qc, Canada).