Intestinal Epithelial Monolayers Derived from Established Murine Colonoids - JoVE

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Monocamadas Epiteliais Intestinais Derivadas de Colonóides Murinos Estabelecidos

Published: June 02, 2023

doi:

Erin Crawford*¹, Heather L. Mentrup*^2,3, Elizabeth A. Novak³, Kevin P. Mollen³

¹Division of Pediatric Gastroenterology, Hepatology and Nutrition,UPMC Children’s Hospital of Pittsburgh, ²Division of Pediatric Surgery,UPMC Children’s Hospital of Pittsburgh, ³Department of Surgery,University of Pittsburgh School of Medicine

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Keywords: Intestinal Epithelial Monolayers Murine Colonoids Basement Membrane Matrix Murine Monolayer Medium Cell Culture Insert DPBS Enzymatic Dissociation Reagent Trypsin

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Intestinal Epithelial Monolayers Derived from Established Murine Colonoids. J. Vis. Exp. (Pending Publication), e200278, doi:10.3791/200278 (2023).

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