Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume

Abstract

Source: Limjunyawong, N. et al,  Instillation and Fixation Methods Useful in Mouse Lung Cancer Research. J. Vis. Exp. (2015).

This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.

Protocol

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Lung Instillation Select a commercial one-inch long 20 g intravenous cannula to use for the intubation. Modify the catheter tip manually bending it to generate a slight curvature at the tip as illustrated in Figure 1. Anesthetize the mouse with a mixture of ketamine (100 mg/kg) and xylazin…

Representative Results

Figure 1: Intravenous catheter with slightly curved tip used for instillation. Figure 2: Sloped 3-ring binder used to support mice for the intubation. This binder set up to hold 3 mice. <p class="jove_conte…

Materials

Laboratory Balance	Ohaus	Adventurer Pro Model AV 313	Other balances can be used if they have a range of 1-300 g
20 g Luer intravenous catheter	Insylte		Several other possible vendors, e.g., Jelco Optiva
500 ml laboratory bottle	Various		Several other possible vendors