Purification of Subpopulations of Extracellular Vesicles: A Technique to Purify Subpopulations of Extracellular Vesicles Using Density Gradient Ultracentrifugation
This video demonstrates the density gradient ultracentrifugation-based purification method to isolate subpopulations of extracellular vesicles suitable for characterization studies.
Protocol
1. Density Gradient Purification Add 1.5 mL of 60% iodixanol (stock solution in water) to the 1.5 mL sucrose/Tris buffer containing EVs to create a final solution containing 30% iodixanol. Pipette up and down several times to mix solution thoroughly. NOTE: Be cautious to avoid losing solution in the pipette tip, as the high iodixanol concentration is quite viscous. Transfer the 30% iodixanol buffer solution containing EVs to the bottom of a 5.5 mL ultracentri…
Purification of Subpopulations of Extracellular Vesicles: A Technique to Purify Subpopulations of Extracellular Vesicles Using Density Gradient Ultracentrifugation. J. Vis. Exp. (Pending Publication), e20632, doi: (2023).