This video describes the technique for recording the epileptiform activity of rhinal cortex-hippocampus organotypic slices ex vivo using electrophysiology or EEG set-up. This helps to study the dynamics and progression of epileptogenesis and screen potential therapeutic targets to treat epilepsy.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of rhinal cortex-hippocampus slices NOTE: The preparation of rhinal cortex-hippocampus slices uses P6-7 Sprague-Dawley rats. Culture setup and medium preparation On the day before the culture, prepare the required media and place them at 4 °C. Prepare d…
Representative Results
Figure 1: Detailed procedure for the preparation of rhinal cortex-hippocampus organotypic slices. (A) Remove the brain from the head and place it in ice-cold GBSS with the dorsal surface faced up. (B) Insert the forceps into the cerebellum. Open the brain through the midline and remove the excess tissue over the hippocampus. (C) With a spatu…
Divulgations
The authors have nothing to disclose.
Materials
Artificial cerebrospinal fluid (aCSF)
Homemade
B-27™ Supplement (50X), serum free
Thermo Fisher Scientific
17504-044
Blades for scalpel handle
Fine Science Tools
10011-00
Bovine Serum Albumin (BSA)
NZYTech
MB04602
5% BSA is used to dilute the primary antibodies. Add 0.5g BSA in 10 mL PBS.