In this video, we demonstrate biolayer interferometry (BLI) technology to detect the intermolecular interactions between ligands and target analytes in real-time. BLI measures changes in the interference pattern of white light reflected off a layer of immobilized ligands on a biosensor surface as they interact with analytes in solution.
Protocol
1. Biosensor hydration and assay set-up Turn the BLItz machine on. Ensure that the machine is connected to the computer through a USB data output port at the back of the machine. On the computer, open the associated software (e.g., BLItz Pro), and click on Advanced Kinetics on the left-hand side of the screen. On the software, type out all appropriate information about the experiment (including the Experiment Name, Description, Sample ID, and Protein Concentratio…
Divulgations
The authors have nothing to disclose.
Materials
BLItz machine
ForteBio
45-5000
Device
Dip and Read Ni-NTA biosensor tray
ForteBio
18-5101
Ready-to-use Ni-NTA biosensors for poly-His-tagged Proteins