In this video, we demonstrate the test to evaluate basophil activation in the presence of a test allergen using flow cytometry.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Sample preparation Collect peripheral blood in 9 mL heparinized tubes and maintain the sample at room temperature (RT) in a rotor until it is required for the experimental protocol. Label 5 mL cytometer tubes for negative controls (2 tubes), positive controls (2 tubes), and different concentrations of allergen/drug (1 t…
Divulgations
The authors have nothing to disclose.
Materials
5 mL Round Bottom Polystyrene Test Tube, without Cap, Nonsterile