A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections

Published: January 31, 2024

Abstract

Source: Chiem, K., et al. A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection. J. Vis. Exp. (150), (2019).

This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. In Vivo Characterization of BIRFLU (Figure 1 and Figure 2) Mouse infection NOTE: Intranasal infection of mice was done as previously described. For a more detailed protocol of IAV infection in vivo using the mouse model of infec…

Representative Results

Figure 1: Schematic representation for the study of BIRFLU in mice. Expression of Nluc and Venus reporter genes was evaluated in mice infected with 1 x 106 PFU of BIRFLU using in vivo or ex vivo imaging. Briefly, on day 1, 5 to 7-week-old female BALB/c mice were mock-infected (1x PBS) or inoculated with 1 x 106 PFU of BIRFLU intranasally. At day 3 post-infe…

Divulgations

The authors have nothing to disclose.

Materials

Five- to seven-week-old female BALB/c mice National Cancer Institute (NCI) 555
Isoflurane Baxter 1001936040 Store at Room temperature
IVIS Spectrum PerkinElmer 124262 This instrument is used for in vivo imaging 
IX81 Motorized Inverted Microscope Olympus Olympus IX81
Living Image 4.7.2 software PerkinElmer This instrument is used for in vivo imaging 
Lumicount Packard This instrument is used for quantifying luciferase activity 
Nano-Glo Luciferase Assay Reagent Promega N1110 This reagent is used to measure Nluc activity. Store at -20 °C
Retiga 20000R Fast1394 Camera Qimaging Retiga 2000R
Scanner HP

Tags

Play Video

Citer Cet Article
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections. J. Vis. Exp. (Pending Publication), e21896, doi: (2024).

View Video