A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E

Abstract

Source: Huang, Z., et al. Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE). J. Vis. Exp. (2019)

This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Basic information of the study group NOTE: The Allergy Information Repository of the State Key Laboratory of Respiratory Disease (AIR-SKLRD) is a large serum bank established inside the Guangzhou Institute of Respiratory Hospital (GIRH). Initiated in the last decade, the AIR-SKLRD has already started collecting and …

Materials

Agnitio BioIC Analyzer	Agnitio Science & Technology(Taiwan, China)	BA-G2000
BioIC Allergen specific-IgE Detection Kit	Agnitio Science & Technology(Taiwan, China)	DR17A12
BioIC Cartrideg Placement plate	Agnitio Science & Technology(Taiwan, China)	T20SET
BioIC Reagent Dispenser	Agnitio Science & Technology(Taiwan, China)	DS-1
Image two-dimensional barcode machine	Agnitio Science & Technology(Taiwan, China)	NLS-HR200
VORTEX-5 Vortex Mixer	Haimen Kylinbell Lab Lastruments Co., Ltd.	VORTEX-5