特里尔社会压力诱发心理应激的测试协议
Summary
本文介绍了诱导参与者的心理压力，这使研究人员能够测量单个参与者或群体之间的心理，生理和神经内分泌反应强调了协议。
Abstract
本文演示了在实验室环境中使用的心理压力协议。协议，使研究人员研究在健康和疾病的应激反应的生物途径是在紧张和焦虑的研究进展的基础。[1]虽然众多的协议，在实验室中诱导应激反应存在，许多忽视提供自然背景或纳入社会和心理压力方面。心理压力协议，荟萃分析表明，特里尔社会压力测试（TSST）的应激激素的反应性。研究，在原来的TSST描述是最有用和最适当的的标准化协议，研究人员试图设计和评估的程序能够诱导可靠的应激反应，在大多数健康志愿者3这些研究人员发现，在心率，血压和几个内分泌应激米的海拔高度arkers的TSST（心理应激）相比，生理盐水注射液（物理应激）。，虽然TSST已被修改，以满足不同的研究小组的需求，它一般由一个到达后的等待期，预期讲话算术准备，演讲表现，口头表现时期，由一个或多个恢复时期。 TSST要求与会者准备并发表讲话，并口头回应一个具有挑战性的算术问题，在一个社会评价的观众。社会评价和不可控性已被确定为关键部件的压力感应的 TSST。使用4十多年来，TSST的目标任务的过程中，有系统地诱导应激反应，以衡量不同反应，焦虑和激活下丘脑 – 垂体 – 肾上腺（HPA）或交感神经 – 肾上腺髓质（SAM）轴1 Researc她一般评估自我报告的焦虑的变化，生理的措施（如心率），和/或神经内分泌指标（如应激激素皮质醇）的TSST。许多研究者通过对应激标志物，如皮质醇和α-淀粉酶（植物神经系统激活的标志）作为替代采血，血液收集技术，以减少混杂强调的唾液取样。除了TSST完成个人所经历的变化，研究人员可以比较不同的治疗组（如临床与健康对照样本）或压力减少干预的有效性的变化1。
Protocol
1。设置所有研究应该由适当的机构审查委员会或人类受试者审查委员会之前，数据收集的批准。 理想的情况下，两房应进行TSST。一个舒适的候车室，应当向与会者提供开始前的TSST在恢复时期。应该使用一个单独的采访室，准备在演讲中，语音性能和数学部分的TSST推出的新颖性和uncontrolability。 安排面试室，使参与者都将面临同盟面试官和位置的视频或录音设备，在参与者?…
Discussion
在这篇文章中，我们演示了如何在一个健康的志愿者进行特里尔社会压力测试。 TSST是一个标准化的实验室社会应激诱导强大而可靠的心理，生理和神经内分泌措施增加。 TSST是一个有用的替代物理压力，如在寒冷的压测试或跑步机上行走，并抄录在评价观众面前表现更自然的心理压力。
的TSST的许多方面可以进行修改，以满足各种研究方案的需求。本文所演示的测量时间点代…
Divulgations
The authors have nothing to disclose.
Acknowledgements
感谢您对杰西卡希斯协议的建议和贡献的有代表性的数据。特别感谢萨布丽娜Blackledge，劳伦科胡特克和Kerisa谢尔顿展示了这一协议。
Materials
| Name of the equipment/Supply | Company | Catalogue number |
|---|---|---|
| BSL Psychophysiology System, Mac OS Including MP36 Data Acquisition Unit and three lead electrocardiogram electrodes | BioPac Systems Inc. | BSLPSY-M |
| State Trait Anxiety Inventory | Mind Garden, Inc. | STAID-B |
| Salivary Cortisol Enzyme Immuno Assay Kit | Salimetrics | 1-3002 |
| 2.0 ml polypropylene vials | Fisher Scientific | 05 40B 146 |
| BioRad Microplate Reader, Model 680, with Microplate Manager software | BioRad | Plate Reader: 168 1000 Software: 1706800 |
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Tags
Trier Social Stress Test ProtocolPsychological StressLaboratory SettingStress ResponseHealth And DiseaseStress Hormone ReactivityTSSTNaturalistic ContextSocial StressPsychological Stress ProtocolsHeart RateBlood PressureEndocrine Stress MarkersSpeech PreparationSpeech PerformanceVerbal Arithmetic Performance
Citer Cet Article
Birkett, M. A. The Trier Social Stress Test Protocol for Inducing Psychological Stress. J. Vis. Exp. (56), e3238, doi:10.3791/3238 (2011).