Materials
Name | Company | Catalog Number | Comments |
Finquel (Tricaine methanesulfonate) | Argent Chemical Laboratories | MS-222 | Larvae anesthesia |
Phenol Red Solution | Sigma-Aldrich | P0290-100 ml | 0.5% in DPBS, cell-culture tested |
Mineral oil, light, white (high purity) | Amresco | J217-500 ml | For needle backfill and volume testing |
TxRed-Dextran, 10,000 MW, lysine fixable | Invitrogen/ Molecular Probes | D-1863 | 1% suspension in 1x PBS/phenol red |
FM4-64FX | Invitrogen/ Molecular Probes | F34653 | 5 mM stock in water |
Methylcellulose | MP Biochemicals | 0215549590 | |
Borosilicate glass capillaries | Drummond Scientific | 3-000-203-G/X | OD 1.14 mm, ID 0.53 mm, 3.5 in length |
Plastic form for mold making | Adaptive Science Tools | TU-1 | |
Nanoject II microinjection unit | Drummond Scientific | 3-000-204 | |
Flaming Brown Micropipette Puller, 3.0 mm wide-trough filament | Sutter Instrument Co. | P-97, FT330B | Needle fabrication |
Student Dumont #5 forceps | Fine Science Tools | 91150-20 | 0.1 x 0.06 mm, needle clipping |
Eyepiece Graticle, 5 mm - 100 divisions | Leica | 10394771 | Needle clipping |
Microforge | Narishige | MF-900 | Needle clipping and fire-polishing |
Tuberculin SlipTip syringe needle | Becton Dickinson | 309626 | 1 ml, 25G 5/8-needle |
Fluoresbrite YG Microspheres (2.0 μm) | Polysciences, Inc. | 18338 | Intestinal motility analysis |
Dissecting Microscope | Leica | S6E | Gavage procedure |
Fluorescence Stereomicroscope | Leica | M205C | Dextran barrier assay |
Confocal microscope | Zeiss | LSM510 | Imaging of dextran in circulation |
References
- Kuhlman, J., Eisen, J. S. Genetic screen for mutations affecting development and function of the enteric nervous system. Dev. Dyn. 236, 118-127 (2007).
- Pack, M., et al. Mutations affecting development of zebrafish digestive organs. Development. 123, 321-328 (1996).
- Wallace, K. N., Pack, M. Unique and conserved aspects of gut development in zebrafish. Dev. Biol. 255, 12-29 (2003).
- Wallace, K. N., Akhter, S., Smith, E. M., Lorent, K., Pack, M. Intestinal growth and differentiation in zebrafish. Mech. Dev. 122, 157-173 (2005).
- Ng, A. N., et al. Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis. Dev. Biol. 286, 114-135 (2005).
- Hama, K., et al. In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters. Am. J. Physiol. Gastrointest. Liver Physiol. 296, 445-453 (2009).
- Carten, J. D., Bradford, M. K., Farber, S. A. Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish. Dev. Biol. 360, 276-285 (2011).
- Rich, A. A new high-content model system for studies of gastrointestinal transit: the zebrafish. Neurogastroenterol Motil. 21, 225-228 (2009).
- Bagnat, M., Cheung, I. D., Mostov, K. E., Stainier, D. Y. Genetic control of single lumen formation in the zebrafish gut. Nat. Cell Biol. 9, 954-960 (2007).
- Bagnat, M., et al. Cse1l is a negative regulator of CFTR-dependent fluid secretion. Curr. Biol. 20, 1840-1845 (2010).
- Shepherd, I., Eisen, J. Development of the zebrafish enteric nervous system. Methods Cell Biol. 101, 143-160 (2011).
- Brugman, S., et al. Oxazolone-induced enterocolitis in zebrafish depends on the composition of the intestinal microbiota. Gastroenterology. 137, 1757-1767 (2009).
- Oehlers, S. H., et al. The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish. Dis. Model Mech. 4, 832-841 (2011).
- Oehlers, S. H., et al. A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents. Dev Dyn. 240, 288-298 (2011).
- Faro, A., Boj, S. F., Clevers, H. Fishing for intestinal cancer models: unraveling gastrointestinal homeostasis and tumorigenesis in zebrafish. Zebrafish. 6, 361-376 (2009).
- Fleming, A., Jankowski, J., Goldsmith, P. In vivo analysis of gut function and disease changes in a zebrafish larvae model of inflammatory bowel disease: a feasibility study. Inflamm. Bowel Dis. 16, 1162-1172 (2010).
- Kanther, M., et al. Microbial colonization induces dynamic temporal and spatial patterns of NF-kappaB activation in the zebrafish digestive tract. Gastroenterology. , (2011).
- Rawls, J. F., Samuel, B. S., Gordon, J. I. Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Proc. Natl. Acad. Sci. U.S.A. 101, 4596-4601 (2004).
- Rawls, J. F., Mahowald, M. A., Goodman, A. L., Trent, C. M., Gordon, J. I. In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut. Proc. Natl. Acad. Sci. U.S.A. 104, 7622-7627 (2007).
- Kanther, M., Rawls, J. F.
Host-microbe interactions in the developing zebrafish. Curr. Opin. Immunol. 22, 10-19 (2010). - Bates, J. M., et al. Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. Dev. Biol. 297, 374-386 (2006).
- Cheesman, S. E., Guillemin, K. We know you are in there: conversing with the indigenous gut microbiota. Res Microbiol. 158, 2-9 (2007).
- Bates, J. M., Akerlund, J., Mittge, E., Guillemin, K. Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota. Cell Host Microbe. 2, 371-382 (2007).
- Camp, J. G., Jazwa, A. L., Trent, C. M., Rawls, J. F. Intronic cis-regulatory modules mediate tissue-specific and microbial control of angptl4/fiaf transcription. PLoS Genetics. , In Press (2012).
- Cheesman, S. E., Neal, J. T., Mittge, E., Seredick, B. M., Guillemin, K. Epithelial cell proliferation in the developing zebrafish intestine is regulated by the Wnt pathway and microbial signaling via Myd88. Proc. Natl. Acad. Sci. U.S.A. 108, 4570-4577 (2011).
- Field, H. A., Kelley, K. A., Martell, L., Goldstein, A. M., Serluca, F. C. Analysis of gastrointestinal physiology using a novel intestinal transit assay in zebrafish. Neurogastroenterol. Motil. 21, 304-312 (2009).
- Westerfield, M. The Zebrafish Book. , 4 edn, University of Oregon Press. (2000).
- Pham, L. N., Kanther, M., Semova, I., Rawls, J. F. Methods for generating and colonizing gnotobiotic zebrafish. Nat. Protoc. 3, 1862-1875 (2008).
- Shen, L., Weber, C. R., Raleigh, D. R., Yu, D., Turner, J. R. Tight junction pore and leak pathways: a dynamic duo. Annu. Rev. Physiol. 73, 283-309 (2011).
- Van Itallie, C. M., et al. The density of small tight junction pores varies among cell types and is increased by expression of claudin-2. J. Cell. Sci. 121, 298-305 (2008).
- Van Itallie, C. M., Anderson, J. M. Measuring size-dependent permeability of the tight junction using PEG profiling. Methods Mol. Biol. 762, 1-11 (2011).
- Watson, C. J., Rowland, M., Warhurst, G. Functional modeling of tight junctions in intestinal cell monolayers using polyethylene glycol oligomers. Am. J. Physiol. Cell Physiol. 281, 388-397 (2001).
- Rodgers, L. S., Fanning, A. S. Regulation of epithelial permeability by the actin cytoskeleton. Cytoskeleton (Hoboken). 68, 653-660 (2011).
- Gonzalez-Mariscal, L., Chavez de Ramirez, B., Cereijido, M. Tight junction formation in cultured epithelial cells (MDCK). J. Membr. Biol. 86, 113-125 (1985).
- Palant, C. E., Duffey, M. E., Mookerjee, B. K., Ho, S., Bentzel, C. J. Ca2+ regulation of tight-junction permeability and structure in Necturus gallbladder. Am. J. Physiol. 245, C203-C212 (1983).
- Holmberg, A., Schwerte, T., Pelster, B., Holmgren, S. Ontogeny of the gut motility control system in zebrafish Danio rerio embryos and larvae. J. Exp. Biol. 207, 4085-4094 (2004).
- Rich, A., et al. Kit-like immunoreactivity in the zebrafish gastrointestinal tract reveals putative. 236, 903-911 (2007).
- Holmberg, A., Olsson, C., Hennig, G. W. TTX-sensitive and TTX-insensitive control of spontaneous gut motility in the developing zebrafish (Danio rerio) larvae. J. Exp. Biol. 210, 1084-1091 (2007).
- Hennig, G. W., Costa, M., Chen, B. N., Brookes, S. J. Quantitative analysis of peristalsis in the guinea-pig small intestine using spatio-temporal maps. J. Physiol. 517 (Pt 2), 575-590 (1999).
- Flynn, E. J. 3rd, Trent, C. M., Rawls, J. F. Ontogeny and nutritional control of adipogenesis in zebrafish (Danio rerio. J. Lipid Res. 50, 1641-1652 (2009).
- Clifton, J. D., et al. Identification of novel inhibitors of dietary lipid absorption using zebrafish. PLoS One. 5, e12386 (2010).
- Jin, S. W., Beis, D., Mitchell, T., Chen, J. N., Stainier, D. Y. Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development. 132, 5199-5209 (2005).
- Berghmans, S., Hunt, J., Roach, A., Goldsmith, P. Zebrafish offer the potential for a primary screen to identify a wide variety of potential anticonvulsants. Epilepsy Res. 75, 18-28 (2007).