Can be difficult to source; also available from Bioptechs
Cover glass/slide gasket
Bioptechs
FCS2 0.75 mm
Fluorescence Microscope
Various
Example setup: Coherent Innova 308C Argon-ion laser, Olympus IX-81 microscope, Olympus PlanApo 100X NA 1.45 objective, Metamorph software
Must have laser excitation, automated xyz stage, automation software capable of scripted imaging and autofocus, optics capable of resolving single fluorescent proteins
EM-CCD Camera
Various
Example setup: Andor Ixon DU-898
Must have sufficiently low noise to detect single fluorescent proteins above background
Hensel, Z., Fang, X., Xiao, J. Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC). J. Vis. Exp. (73), e50042, doi:10.3791/50042 (2013).