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Chapters
- 00:05Introduction
- 01:34Fabricating the Master Wafer and Preparing the Microfluidic Device
- 03:46Making the PDMS-Coated Glass Slide and Bonding the Microfluidic Device
- 05:24Surface Functionalization of the Microfluidic Device
- 06:48Liposome Production
- 07:33Results: Production of Monodispersed Liposomes and Subsequent pH-Triggered Liquid-Liquid Phase Separation of pLL/ATP Inside Liposomes
- 09:02Conclusion
Den nåværende protokollen beskriver oktanolassistert liposommontering (OLA), en mikrofluidisk teknikk for å generere biokompatible liposomer. OLA produserer monodispergerte liposomer i mikronstørrelse med effektiv innkapsling, noe som muliggjør umiddelbar eksperimentering på brikken. Denne protokollen forventes å være spesielt egnet for syntetisk biologi og syntetisk celleforskning.
Tags
On-chip Liposome AssemblyOLAMicrofluidic PlatformSynthetic BiologyArtificial CellsSelf-organizationCell-mimicking AssembliesMonodispersed LiposomesHigh-throughputEncapsulation EfficiencyBiological ReactionsVesicle DynamicsBiomolecular CondensatesMembrane InteractionsDrug ScreeningAntimicrobial TestingSurface FunctionalizationPhotolithography
