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Zebrafish Brain Ventricle Injection
 
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Zebrafish Brain Ventricle Injection

Article DOI: 10.3791/1218-v 08:29 min April 6th, 2009
April 6th, 2009

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résumé

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After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.

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Zebrafish Brain Ventricle Embryonic Brain Development Cerebrospinal Fluid Neuroepithelium Ventricle Formation Visualization Fluorescent Dye Brightfield Microscopy Fluorescent Microscopy Photoshop Neuroepithelial Shape Changes Brain Tissue Brain Morphogenesis Mutants
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