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Dendra2 Photoswitching through the Mammary Imaging Window
 
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Dendra2 Photoswitching through the Mammary Imaging Window

Article DOI: 10.3791/1278-v • 09:13 min • June 5th, 2009
June 5th, 2009 •
1,2, 1, 1, 1,2, 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, 3Hubrecht Institute-KNAW and University Medical Center Utrecht

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Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

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Intravital Microscopy Breast Tumors Mice Rats Single-cell Resolution Metastatic Behavior Migration Invasion Intravasation Angiogenesis Immune Cells Response Mammary Carcinomas Mammary Imaging Window (MIW) Imaging Parameters Dendra2 Photoswitching In Vivo Imaging Protocol Manufacturing MIW Tumor Cells Imaging Custom Built Imaging Box Metastatic Behavior
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