Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:55Introduction
- 01:49An Optical Projection Tomography Microscope
- 04:51Preparing Tissue for Tomographic imaging
- 09:30Absorption Reconstructions
- 11:36Fluorescent Reconstructions
- 15:44Conclusion
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
Tags
Born NormalizationFluorescence Optical Projection TomographyWhole Heart ImagingThree-dimensional Imaging TechniqueBiologie du développementGene Expression StudiesOptical TransparencyBenzyl AlcoholBenzyl BenzoateBABBMurray’s Clear SolutionClearing ProcessScattering ContributionAbsorption ContributionFluorescence DistributionImage ArtifactsQuantification ErrorsExogenous Contrast AgentsEndogenous Contrast
