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Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
Journal JoVE
Biologie
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Journal JoVE Biologie
Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
DOI:

10:27 min

August 25, 2009

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Chapitres

  • 00:05Titre
  • 00:36Introduction
  • 01:19Patterning Gold Microelectrodes Using Lithography
  • 03:27Preparation of the Self-assembled Monolayer (SAM)
  • 05:36Polymerization of Tubulin into Microtubules
  • 07:09Patterning the Microtubules using Electrophoresis
  • 09:25Representative Results of Microtubule Patterning on SAM-coated Electrodes
  • 09:53Conclusion

Summary

Traduction automatique

We present a procedure for forming a poly(ethylene glycol) self-assembled monolayer (PEG-SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single step and prevents biofouling on silicon and gold surfaces. Electrophoresis is then used for patterning biomolecules down to the nanoscale.

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