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Chapitres
- 00:00Titre
- 00:09Introduction
- 00:30Viral Transduction of Mouse Embryonic Fibroblasts
- 01:25Doxycycline Induced Reprogramming
- 03:18Immunocytochemical Analysis of Transduction Efficiency
- 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
- 07:39Immunocytochemical Analysis for Pluripotency
- 09:14Pluripotency Marker Analysis
- 10:31Conclusion
Stemgent Dox Đndüklenebilir Fare TF lentivirüs Set bağlı pluripotent kök (IPS) hücreleri fare embriyonik fibroblastlar (MEFS) yeniden programlamak. Burada fare reprogramming transkripsiyon faktörleri Oct4 DOX indüklenebilir ifade için protokol göstermek, Sox2, Klf4 ve c-Myc ifade ortak MES pluripotency belirteçlerinin iPS koloniler oluşturmak için.
Tags
Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set
