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Chapitres
- 00:06Assembling handing drops differentiation culture
- 00:08Collect undifferentiated ES cells
- 02:24Prepare materials
- 02:57Assemble hanging drop culture
- 04:34Collecting differentiated ES cells form hanging drops
- 06:05Starting EB culture
- 06:53EB dissociation and infection
- 11:25Preparation of ES cell-derived progenitors for FACS
- 15:18Transplantation
- 16:19Discussion
Questa dettagli del protocollo la derivazione di cellule staminali ematopoietiche trapiantabili da topo cellule staminali embrionali (ESC) e la loro successiva iniezione in topi irradiati letalmente destinatario. In breve, ESC si differenziano come corpi embrionali, che sono poi infettati con HoxB4 retrovirali e co-coltura con cellule stromali OP9 e citochine ematopoietiche.
Tags
Stem CellsHematopoietic Stem CellsMurine Embryonic Stem CellsSelf-renewalDifferentiated ProgenyPluripotentDifferentiative AbilityClinically Useful Cell TypesVideo ProtocolHematopoietic MalignanciesHematopoietic DisordersHSC TherapyAlternative Source Of HSCBaselineHuman ESC IsolationEmbryoid Bodies (EBs)Serum Pre-screenedHematopoietic DifferentiationRetroviral HoxB4OP9 StromaBone Marrow Stromal Cell LineCytokines
