Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:42Introduction
- 01:17Pool Design and Oligo Recovery
- 03:53Co-immunoprecipitation of the Oligo Pool with an RNP of Interest
- 07:50Labeling the Samples and Hybridizations to the Microarray
- 10:50Mapping RNPs on Pre-mRNAs Representative Results
- 11:56Conclusion
Tags
Rapid High-throughput MethodMapping RibonucleoproteinsRNPsHuman Pre-mRNACo-immunoprecipitateRNA Binding ProteinsSplicing FactorIn Vitro ApproachOligonucleotide PoolMolecular SelectionTwo Color Array StrategyEnrichmentHigh-resolution MapsSequence-specific DeterminatesStructural DeterminatesRibonucleoprotein BindingUnique AdvantageSampling BiasIP TechniquesSELEX TechniquesFlexibility
