Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:31Introduction
- 02:14Administering Tamoxifen by Oral Gavage
- 04:17Whole Mount Microscopy of E12.5 Embryos
- 05:10Craniotomy and Adult Whole Mount Microscopy
- 08:03Explant Preparation and Micro-Dissection of the Ventral Mesencephalon from E12.5 Embryos
- 09:59Representative Results for Wnt1 Fate-Mapping
- 12:11Conclusion
Genetic Inducible Fate Mapping (GIFM) marks and tracks cells with fine spatial and temporal control in vivo and elucidates how cells from a specific genetic lineage contribute to developing and adult tissues. Demonstrated here are the techniques required to fate map E12.5 mouse embryos for epifluorescent and explant analysis.
Tags
Genetic Inducible Fate MappingFate MapsMarking And Tracking Cells In VivoProgenitorsSpecific StructuresCell TypesGenetic Inducible Fate Mapping (GIFM)Gene ExpressionCell FateCell BehaviorsX-CreER LinesBacteriophage ProteinCre RecombinaseCreERTModified Estrogen Receptor Ligand Binding DomainTamoxifenRecombinationLoxP SitesStop CassetteReporter GeneGFPMice BreedingConditional Reporter Allele
