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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

DOI:
10.3791/1792-v

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07:55 min

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May 02, 2010

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, , , ,
1Department of Medical Neurobiology,Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

Chapitres

  • 00:00Titre
  • 00:11Introduction
  • 00:50Egg Handling and Preparing for Electroporation
  • 02:45Windowing and Electroporation
  • 04:56Spinal Cord Open-Book Preparation
  • 06:53Chick Neural Tube in Ovo Electroporation Representative Results
  • 07:25Conclusion

Summary

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This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.

Tags

Axonal PathwaysGenetically Defined NeuronsChick Neural TubeIn Ovo ElectroporationEnhancer ElementsReporter GenesTracking Axonal ProjectionSpinal InterneuronsGerm Line-targeted Reporter GenesBilateral LabelingIpsi- And Contralaterally Projecting AxonsUnilateral ElectroporationCentral Nervous SystemHandling EggsInjectionTungsten ElectrodesElectrical PulsesPulse GeneratorIncubatorSpinal Cord PreparationWhole Mount Antibody StainingConfocal Microscopy

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