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Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
 
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Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography

Article DOI: 10.3791/1846-v • 09:24 min • October 29th, 2010
October 29th, 2010 •
*1, *1,2, *3, *1, 3, 1,3
1School of Biology, Georgia Institute of Technology, 2Department of Molecular Pharmacology, RWTH Aachen University, 3School of Chemistry and Biochemistry, Georgia Institute of Technology
* These authors contributed equally

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Evaluating two-dimensional (2D) crystallization trials for the formation of ordered membrane protein arrays is a highly critical and difficult task in electron crystallography. Here we describe our approach in screening for and identifying 2D crystals of predominantly small membrane proteins in the range of 15 – 90kDa.

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Two-dimensional Crystallization Trials Small Membrane Proteins Structural Biology Studies Electron Crystallography Transmission Electron Microscopy (EM) Cryo-EM Large And Ordered 2D Arrays Crystallization Conditions Morphology And Membrane Size Order And 2D Crystal Dimensions CCD Cameras Online-Fourier Transforms Proteoliposomes Reconstitution By Dialysis Monolayers Native 2D Crystals Ordered Arrays Of Soluble Proteins
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