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Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE
 
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Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Article DOI: 10.3791/200472-v • 05:11 min • June 2nd, 2023
June 2nd, 2023 •
1, 1,2, 2, 1,2, 1,2
1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, 2Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

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MtHyPer7 Ratiometrik Biyosensör Mitokondriyal Peroksit Canlı Maya Hücreleri Mitokondriyal Disfonksiyon Nörodejeneratif Bozukluklar Kas-İskelet Sistemi Bozuklukları Kanser Yaşlanma Genetik Olarak Kodlanmış Biyosensör Minimal İnvaziv Hidrojen Peroksit (H2O2) Mitokondri Hedefli HyPer7 (mtHyPer7) Dairesel Permütasyonlu Floresan Protein OxyR Protein Alanı CRISPR-Cas9 Sistemi İşaretsiz Sistem Maya Genom Entegrasyonu Dönen Disk Konfokal Mikroskop Sistemi Kantitatif Analiz Oksidatif Stres Uzay-Zamansal Bilgi
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