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Biochemistry

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JoVE Journal Biochemistry
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE
 
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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE

Strategies for Expressing and Purifying Solute Carrier Transporters

Article DOI: 10.3791/200669-v • 01:12 min • September 29th, 2023
September 29th, 2023 •
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1Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

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High-Throughput Expressie Zuivering Menselijke Opgeloste Stof Dragers Structurele Studies Biochemische Studies Membraantransporters Endogene Substraat Exogene Substraten Therapeutische Doelen Markers Van Ziekte Farmaceutica Drug Discovery Projecten Beperkte Structurele Kennis Beperkte Functionele Kennis Beperkte Fysiologische Kennis Expressie En Zuivering Moeilijkheden Milligram Hoeveelheden Codon-geoptimaliseerde gensequenties Construct Design High-throughput Expressie Behoud van structurele integriteit behoud van biochemische activiteit eukaryote celexpressie affiniteitszuivering grootte-uitsluitingschromatografie structuurbepaling met hoge resolutie transportstudies, Betrokkenheidstests voor kleine moleculen in-vitroscreening met hoge doorvoer
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