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Isolation and Magnetic Separation of Murine CD34⁺ Stem Cells from the Vascular Wall

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Detection of Intracellular Calcium Signaling in the Magnetically Isolated Vascular Wall CD34⁺ Stem Cells

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Médecine

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Journal JoVE Médecine

Detection of Intracellular Calcium Signaling in the Magnetically Isolated Vascular Wall CD34⁺ Stem Cells

Detection of Intracellular Calcium Signaling in the Magnetically Isolated Vascular Wall CD34⁺ Stem Cells

Isolation and Magnetic Separation of Murine CD34⁺ Stem Cells from the Vascular Wall

DOI:

10.3791/201056-v

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01:15 min

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December 22, 2023

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Chuting Han, Cheng Xie, Qingya Dang, Xiaolin Zhang, Chunshu Li, Yan Yang, Jun Cheng, Pengyun Li

¹Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research,Southwest Medical University

Tags

Immunomagnetic Isolation Vascular Wall-resident CD34+ Stem Cells Purification Flow Cytometry Functional Identification Immunofluorescence Staining Ca2+ Imaging Murine Aorta And Mesenteric Artery Adventitia Cell Culture Multipotency Differentiation ATP Caffeine Thapsigargin

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Magnetic Separation of Vascular Wall-Resident CD34⁺ Stem Cells Isolated from Murine Aortal and Mesenteric Arteries for Cardiovascular Studies

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