Journal
/
Immunologie et infection
/
Göç İnsan T Lenfosit İzolasyon, Kültür ve Analiz In Vitro</em
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Please note that all translations are automatically generated. Click here for the English version.

Göç İnsan T Lenfosit İzolasyon, Kültür ve Analiz In Vitro</em

DOI:
10.3791/2017-v

•

08:39 min

•

June 01, 2010

•

,
1Center for Vaccine Biology and Immunology,University of Rochester

Chapitres

  • 00:00Titre
  • 00:37Introduction
  • 01:31Isolation of Human T Lymphocytes
  • 02:57Culture of Human T Lymphocytes
  • 05:04In Vitro Lymphocyte Migration Assay
  • 06:15Capturing an Image Sequence Using NIS Elements Software
  • 07:20Representative Results
  • 08:12Conclusion

Summary

Traduction automatique

Traduction automatique

T lenfosit göç homing lenfoid organlar, damar çıkış sırasında oluşur ve periferik dokularda girmeden. Burada, T lenfosit göçü analiz etmek için kullanılan bir protokol tarif<em> In vitro</em>.

Tags

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Primer insan T hücrelerinin piggyBac Transposon Sistem Modifikasyon
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Fenotipik Analizi ve İnsan Cilt lenfosit izolasyonu<em> Ex Vivo</em> Hücre Kültürü
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Fare Naif CD4<sup> +</sup> T Hücre İzolasyonu ve<em> In vitro</em> T hücre alt içine Farklılaşma
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Iltihaplı Dermisinde içinde görüntüleme CD4 + T hücre Geçiş Göç
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

İnsan Tüm Kan Natural Killer Hücre Parçalayıcı Faaliyet Akışı Sitometrik Analizi
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

İnsan Endoservikal Gamma Delta T Hücreleri İzolasyon ve Akım Sitometrisi Analizi
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Bir kullanma Lenfosit Ekstravazasyonunun analizi<em> İn Vitro</em> İnsan Kan-beyin bariyeri Model
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

İnsan monosit kemotaksisi Vitro ve In Vivo kaçakçılığı fare lenfosit miktarının
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Yalıtım ve fare ve insan alveoler makrofajlar Vitro kültürü
check_url/fr/2017?article_type=v&slug=human-t-lymphocyte-isolation-culture-analysis-migration

Yamultma akış kaynaklı geçiş antikorundan marjinal bölge B hücrelerinin in Vitro analizine

Read Article