Microdissection of Zebrafish Embryonic Eye Tissues

Liyun Zhang; Yuk Fai Leung

doi:10.3791/2028

Journal

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Biologie

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Microdissection of Zebrafish Embryonic Eye Tissues

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Biologie

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Journal JoVE Biologie

Microdissection of Zebrafish Embryonic Eye Tissues

DOI:

10.3791/2028-v

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09:01 min

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June 27, 2010

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Liyun Zhang, Yuk Fai Leung

¹Department of Biological Sciences,Purdue University

Chapitres

00:00Titre
01:03Introduction
01:36Materials and Preparation for Microdissection
04:14Brain Removal, Eye Exposure and Retinal Dissection
05:57RPE-Attached Retinal Dissection
07:12Dissection Integrity and RNA Expression Profiles of Whole Retina and RPE
08:10Conclusion

Summary

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This article describes an approach to microdissect zebrafish retinas with and without retinal pigment epithelium attached, from one to three days postfertilization embryos.

Microdissection of Zebrafish Embryonic Eye Tissues

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Chapitres

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