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差分成像生物结构与双共振相干反斯托克斯拉曼散射（CARS）
Journal JoVE
Biologie
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Journal JoVE Biologie
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
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差分成像生物结构与双共振相干反斯托克斯拉曼散射（CARS）

DOI:
10.3791/2085-v

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12:56 min

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October 17, 2010

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,
1Center for Biophotonics,University of California, Davis, 2Department of Internal Medicine,University of California, Davis

Chapitres

  • 00:00Titre
  • 00:46Introduction
  • 01:21Overview of the DR-CARS Microscopy System
  • 05:22The Generation of CARS and Doubly-resonant CARS Signals
  • 07:02Sample Preparation
  • 11:16Imaging Results
  • 12:20Conclusion

Summary

Traduction automatique

Traduction automatique

结合三个单一波长的短脉冲激光器是用来产生相干反斯托克斯拉曼散射（CARS）和双谐振车（DR车）。这些信号之间的区别，否则很难察觉连贯的拉曼信号增强的灵敏度，使弱拉曼散射成像。

Tags

Differential ImagingBiological StructuresDoubly-resonantCoherent Anti-stokes Raman Scattering (CARS)Coherent Raman ImagingLabel-free Optical ImagingMolecular SpecificitySensitivityFluorescenceWeak ConcentrationsRaman-active MoleculesSignal AmplificationPulsed LasersCoherent Raman Scattering SignalsCoherent Anti-stokes Raman Scattering (CARS)3-color CARSFour-wave Mixing (FWM)Doubly-resonant CARS (DR-CARS)Constructive InterferenceTuning LasersCH Stretch VibrationCD Stretching Vibration

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