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Journal / Neuroscience / Axoplasm Isolation from Rat Sciatic Nerve
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JoVE Journal Neuroscience
Axoplasm Isolation from Rat Sciatic Nerve
 
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Axoplasm Isolation from Rat Sciatic Nerve

Article DOI: 10.3791/2087-v • 05:29 min • September 24th, 2010
September 24th, 2010 •
1, 1, 1
1Department of Biological Chemistry, Weizmann Institute of Science

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We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.

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Axoplasm Isolation Rat Sciatic Nerve Peripheral Nerve Biochemical Studies Protocol Dissection Connective Tissue Hypotonic Medium Myelin Non-axonal Structures Axon-enriched Material Proteomic Characterization Biochemical Characterization Axonal Components
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