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Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
 

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Article DOI: 10.3791/2288-v • 12:10 min • February 27th, 2011
February 27th, 2011 •
1,2, *1, *1, 1, 1, 1,2
1Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania
* These authors contributed equally

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résumé
Automatic Translation

Stable isotopic profiling by gas chromatography mass spectrometric analysis of intermediary metabolic flux is described in the nematode, Caenorhabditis elegans. Methods are detailed for assessing isotopic enrichment in carbon dioxide, organic acids, and amino acids following isotope exposure either during development on agar plates or during adulthood in liquid culture.

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Stable Isotopic Profiling Intermediary Metabolism Metabolic Flux Caenorhabditis Elegans Nematode Growth Media Agar Plates Pharmacologic Treatments High Performance Liquid Chromatography (HPLC) Mass Spectrometry Carbon Dioxide Glycolysis Pyruvate Metabolism Tricarboxylic Acid Cycle Isotope Exposure Time Bacterial Clearing Protocols Worm Disruption Methods
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