Journal
/
Biologie
/
在开发和成年阶段的中介代谢通量的稳定同位素分析线虫</em
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Please note that all translations are automatically generated. Click here for the English version.

在开发和成年阶段的中介代谢通量的稳定同位素分析线虫</em

DOI:
10.3791/2288-v

•

12:10 min

•

February 27, 2011

•

, , , , ,
1Department of Pediatrics,The Children’s Hospital of Philadelphia, 2Department of Pediatrics,University of Pennsylvania

Chapitres

  • 00:05Titre
  • 01:51Preparing Worm Samples for Metabolite Quantitation
  • 04:37Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
  • 07:11Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
  • 08:39Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
  • 11:40Conclusion

Summary

Traduction automatique

Traduction automatique

稳定同位素分析气相色谱质谱中介代谢通量的质谱分析中所述的线虫，<em>线虫</em>。详细的评估中的二氧化碳，有机酸，氨基酸和同位素暴露在琼脂平板上的发展，或在液体培养成年的同位素富集的方法。

Tags

Stable Isotopic ProfilingIntermediary MetabolismMetabolic FluxCaenorhabditis ElegansNematode Growth Media Agar PlatesPharmacologic TreatmentsHigh Performance Liquid Chromatography (HPLC)Mass SpectrometryCarbon DioxideGlycolysisPyruvate MetabolismTricarboxylic Acid CycleIsotope Exposure TimeBacterial Clearing ProtocolsWorm Disruption Methods

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

产生稳定的转基因C.使用微量注射线虫

分离和<em在体外激活线虫精子

实心板为基础的饮食限制在线虫</em

时间推移显微镜早期胚胎发育线虫

基本的<em>线虫</em>方法：同步和观察

前列腺素的提取与分析<em>线虫</em

栽培<em>秀丽隐杆线虫</em>在三个维度实验室

线虫的核苷酸和蛋白质提取联合

线虫的耗氧率测定

卡诺尔哈布炎细胞外囊泡的纯化与分析

Read Article