Journal
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Immunologie et infection
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Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

DOI:
10.3791/2383-v

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17:30 min

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January 15, 2011

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, , ,
1Department of Systems Biology,Danish Technical University, 2Department of Biology,University of Copenhagen

Chapitres

  • 00:00Titre
  • 00:08Introduction
  • 00:33Glueing of the Flow Cells
  • 03:59Assembly of Bubble Traps and Attachment of Tubings
  • 05:22Attachment of Flow Cells to the Bubble Traps
  • 08:38Sterilization of the System
  • 10:07Washing the System
  • 11:26Inoculating the Flow Cell
  • 12:19Incubation of the System
  • 16:19Representative Results

Summary

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Protocol describing the application of a flow cell system for growing and analyzing microbial biofilms for Confocal Laser Scanning Microscopy (CLSM).

Tags

Pseudomonas AeruginosaSaccharomyces CerevisiaeBiofilmFlow CellsMicrobial CommunitiesAltered Physiological PropertiesPathological PropertiesTransparent SubstratumNon-destructive ExaminationReal-time StudiesFlow Cell Model SystemReproducibilityGrowth ChamberNutrientsOxygen SupplyPeristaltic PumpWaste ContainerContinuous Supply Of NutrientsAdministration Of AntibioticsMinimal DisturbanceShear StressBubble Trapping DeviceConfocal Laser Scanning Microscopy (CLSM)3D Information

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