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Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
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Journal JoVE Bio-ingénierie
Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

DOI:
10.3791/2413-v

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10:13 min

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December 22, 2010

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1Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences Oncology Center and Institute for NanoBioTechnology,Johns Hopkins University

Chapitres

  • 00:05Titre
  • 01:33Standard Photolithography for Micropatterned Stamp Fabrication
  • 04:08Hyaluronic Acid Micropatterning
  • 07:57Cell Culture on HA Micropatterned Surfaces
  • 08:54Representative Results
  • 09:57Conclusion

Summary

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A novel approach that allows the high-resolution analysis of cancer cell interactions with exogenous hyaluronic acid (HA) is described. Patterned surfaces are fabricated by combining carbodiimide chemistry and microcontact printing.

Tags

Micropatterned SurfacesHyaluronic AcidCancer CellsTumor MicroenvironmentECM ComponentsHA-CD44 InteractionTumor ProgressionCell MigrationMetastatic SpreadGlycosaminoglycanChemical ModificationsImmobilization Of HACell AdhesionFunctionalized SurfacesMicrocontact Printing (uCP)Cancer Cell Interactions

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