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Chapitres
- 00:05Titre
- 00:50Introduction
- 02:52Microfluidic Device Fabrication
- 05:45Formation of Uniform Tumor Spheroids using the Hanging Drop Method
- 07:04Introduction of Spheroids into the Microfluidic Device
- 09:18Introduction of Apoptosis-detecting Agent into the Microfluidic Device
- 10:50Introduction of Therapeutic Agents into the Microfluidic Device
- 11:59Time Lapse Microscopy and Estimation of Drug Diffusivity Coefficients
- 14:25Representative Results
- 15:30Concluding Remarks
Tags
Microfluidic DeviceTumor MicroenvironmentIn VitroDrug DeliverySystemic ClearanceHeterogeneousVasculatureNutrientsViable CellsQuiescent CellsNecrotic CellsCancer DrugsPenetrationMonolayersIn Vitro ModelPDMSSoft LithographyMulticellular Tumor SpheroidsHanging Drop MethodRectangular ChambersContinuous Medium PerfusionLinear GradientsFluorescent StainsApoptosis VariabilityChemotherapeutic Drug DoxorubicinDiffusion CoefficientCell Lines
