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Heterokaryon Technique for Analysis of Cell Type-specific Localization
 
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Heterokaryon Technique for Analysis of Cell Type-specific Localization

Article DOI: 10.3791/2488-v • 09:31 min • March 11th, 2011
March 11th, 2011 •
*1, *1, 1
1Department of Chemistry and Biochemistry, Worcester Polytechnic Institute- WPI
* These authors contributed equally

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A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.

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Heterokaryon Technique Cell Type-specific Localization Subcellular Trafficking Nucleocytoplasmic Shuttling Proteins Cellular Functions Nuclear Import/export Transcriptional Regulation Apoptosis Cell Division Differentiation Transformation Protein Activities Cellular Oncogenesis Normal Primary Cells Transformed Cells Altered Localization Pathways Drug Targeting Protein Trafficking Shuttling Activity Mammalian Cells Heterokaryon Fusions Fluorescence Microscopy
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