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Bioenergetic Profile Experiment using C2C12 Myoblast Cells
 

Bioenergetic Profile Experiment using C2C12 Myoblast Cells

Article DOI: 10.3791/2511-v • 07:20 min • December 6th, 2010
December 6th, 2010 •
1, 2, 3, 3, 3, 3
1Buck Institute for Age Research, Novato, CA, 2Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham - UAB, 3Seahorse Bioscience, North Billerica, MA

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A description of a method for profiling mitochondrial function in cells is provided. The mitochondrial profile generated provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity.

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Bioenergetic Profile Experiment C2C12 Myoblast Cells Cellular Metabolism Mitochondrial Dysfunction Obesity Diabetes Aging Cancer Cardiovascular Function Safety Toxicity Substrate Uptake Oxygen Glucose Fatty Acids Glutamine Energy Conversion Enzymatically Controlled Oxidation And Reduction Reactions ATP Production Heat Release Lactate Production CO2 Release Extracellular Environment Physiological State Of Cells Oxygen Consumption Rate (OCR) Glycolysis Lactate Production Protons Release Acidification Rate (ECAR) Seahorse Cell Culture Plates
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