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Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

DOI:
10.3791/2564-v

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11:57 min

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May 17, 2011

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, , ,
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology,The Salk Institute for Biological Studies

Chapitres

  • 00:05Titre
  • 02:09Preparation of Organotypic Cerebellar Cultures
  • 05:53Fas Treatment and Staining of Cerebellar Slices
  • 09:49Examples of Cerebellar Slice Cultures
  • 10:49Conclusion

Summary

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This method describes the generation of organotypic cerebellar cultures and the effect of certain apoptotic stimuli on the viability of different cerebellar cell types.

Tags

Organotypic Cerebellar CulturesApoptotic ChallengesDetectionNeurosciencesOrganotypic CulturesPreparationSlicesStaining ProcedureBrain PartsHippocampusCerebellumCellular OrganizationNeuronal NetworksPurkinje CellsDissociated Primary CultureDevelopmental FeaturesIn Vitro StudiesElectrophysiological ExperimentsPharmacological ExperimentsApoptotic StimuliFas LigandTUNEL StainingCell Death Measurement

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