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器官型小脑文化：细胞凋亡的挑战和检测
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
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器官型小脑文化：细胞凋亡的挑战和检测

DOI:
10.3791/2564-v

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11:57 min

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May 17, 2011

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, , ,
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology,The Salk Institute for Biological Studies

Chapitres

  • 00:05Titre
  • 02:09Preparation of Organotypic Cerebellar Cultures
  • 05:53Fas Treatment and Staining of Cerebellar Slices
  • 09:49Examples of Cerebellar Slice Cultures
  • 10:49Conclusion

Summary

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Traduction automatique

这种方法描述了一代器官小脑文化和不同的小脑细胞类型的可行性某些凋亡刺激的效果。

Tags

Organotypic Cerebellar CulturesApoptotic ChallengesDetectionNeurosciencesOrganotypic CulturesPreparationSlicesStaining ProcedureBrain PartsHippocampusCerebellumCellular OrganizationNeuronal NetworksPurkinje CellsDissociated Primary CultureDevelopmental FeaturesIn Vitro StudiesElectrophysiological ExperimentsPharmacological ExperimentsApoptotic StimuliFas LigandTUNEL StainingCell Death Measurement

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