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小鼠小肠移植
Journal JoVE
Biologie
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Journal JoVE Biologie
Small Bowel Transplantation In Mice
DOI:

36:30 min

August 20, 2007

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Chapitres

  • 00:03Titre
  • 00:16Introduction
  • 01:18Harvesting Small Bowel from Donor Mouse
  • 01:201. performing Tail Pinch to Ensure Mouse is Anesthetized
  • 02:462. clearing away tissue to allow ligation of small bowel
  • 04:213. cleaning out small bowel contents before ligating ileum
  • 04:504. ligating ileum
  • 05:205. exposing and ligating mesenteric vessels
  • 06:186. exposing mesenteric artery which will be cut when small bowel is
  • 06:427. exposing portal vein
  • 07:288. ligating branch of portal vein
  • 07:589. exposing other side of mesenteric artery
  • 08:0910. locating inferior vena cava
  • 08:1511. injecting heparin into the inferior vena cava
  • 08:3612. cutting mesenteric artery
  • 08:5413.clearing away tissue from mesentaric artery
  • 09:2314. cutting jejunal end of ligated small bowel
  • 09:3715. cutting away small bowel mesenteric tissue
  • 10:2716. cutting away mesenteric tissue until the portal vein is reached
  • 10:5617. cutting ileal end of ligated small bowel
  • 11:1918. cutting away mesenteric tissue
  • 11:3419. cutting portal vein
  • 11:5720. harvesting donor small bowel
  • 12:09Preparing Recipient Mouse for Small Bowel Transplantation
  • 13:201. exposing inferior vena cava and aorta
  • 13:292. clearing away tissue in order to apply ligatures
  • 14:353. applying ligatures to inferior vena cava and inferior aorta
  • 15:03Connecting Donor Small Bowel Circulation to Recipient Circulation
  • 15:151. placing donor small bowel in recipient mouse
  • 15:56puncturing inferior vena cava for veinotomy
  • 16:173. performing veinotomy on inferior vena cava
  • 16:314. repeat procedure for aorta
  • 17:005. washing away blood from vessels with saline
  • 17:156. suturing donor mesenteric artery to recipient aorta
  • 19:597. suturing donor portal vein to recipient inferior vena cava
  • 22:398. final suture for anastamosis of donor portal vein to recipient i
  • 23:019. flipping donor small bowel in order to finish suture of mesenter
  • 23:3510. suturing donor mesenteric artery to other side of aorta
  • 24:5311. final suture for anastamosis of donor mesenteric artery to reci
  • 25:5612. releasing ligatures to open circulation to donor small bowel
  • 26:47Connecting Donor Small Bowel to Recipient Small Bowel
  • 27:201. clamping ileal end of donor small bowel
  • 27:392. cutting recipient jejunum
  • 27:503. cutting ileal end of donor small bowel
  • 28:044. suturing one side of donor ileum to recipient jejunum
  • 29:105. flipping small bowel clamps in order to suture other side of ana
  • 30:206. removing clamps
  • 31:147. creating stoma for jejunal end of donor small bowel
  • 32:028. suturing jejunal end of donor small bowel to stoma
  • 32:409. cutting ligature to jejunal end of donor small bowel
  • 33:14Sewing up Abdominal Cavity
  • 35:32Conclusion

Summary

Traduction automatique

小鼠小肠移植模型已经认识到作为一个重要工具,以研究免疫排斥反应和屏幕上的新的免疫抑制药物的mechanismes。然而,这种模式是有限的使用,因为所涉及的技术是一个非常技术上的挑战。现在,我们引进的改性技术。

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