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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
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Biologie
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Journal JoVE Biologie
Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

DOI:
10.3791/2625-v

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08:32 min

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March 24, 2011

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,
1Human Genetics,University of Michigan

Chapitres

  • 00:05Titre
  • 01:10Mounting Worm Embryos on Agar “Worm Pads”
  • 02:53Setting Up Nomarski DIC Optics for 4D Data Set
  • 05:56Making a GFP Movie
  • 07:17Time-lapse Movies of Early Cell Divisions
  • 07:55Conclusion

Summary

Traduction automatique

Traduction automatique

The C. elegans embryo is a powerful system for studying cell biology and development. We present a protocol for live imaging of C. elegans embryos utilizing DIC optics or fluorescence using readily available epifluorescent microscopes and open-source software.

Tags

ImagingC. Elegans EmbryosEpifluorescent MicroscopeOpen Source SoftwareTime-lapse ImagingLiving CellsFluorescent-labeled Reporter ProteinsDifferential Interference Contrast MicroscopyDevelopmental RegulationsCell BiologyDevelopmentCaenorhabiditis Elegans EmbryoCell LineageGenetic ManipulationForward GeneticsReverse GeneticsRNAi-mediated InterferenceTargeted MutagenesisTransgenic AnimalsFluorescently Tagged ProteinsCompound Epifluorescent Microscope

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