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Bioengineering

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JoVE Journal Bioengineering
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
 
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Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Article DOI: 10.3791/2640-v • 04:24 min • February 13th, 2011
February 13th, 2011 •
1, 2, 1, 3, 2
1Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, 2Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, 3HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School

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We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.

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Cell Rolling Trajectories Asymmetric Receptor Patterns Label-free Separation Analysis Of Cells Lateral Displacement Continuous-flow Separation Receptor Expression Cell Phenotypes Adhesion Modulation Cell Rolling Adhesion P-selectin Receptors Microcontact Printing Gold-coated Slides Flow Chamber HL60 Cells PSGL-1 Ligand Inclined Edges Pattern Edges Edge Tracking Length
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