Journal
/
Biologie
/
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

DOI:
10.3791/270-v

•

13:50 min

•

August 30, 2007

•

, ,
1Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital

Chapitres

  • 00:00Creating channel inlet and outlet
  • 00:06Introduction
  • 01:27Microfabrication of microfluidic device
  • 01:45Preparation of PDMS mixture
  • 02:42Patterning PDMS molds
  • 03:36Removing bubbles by vacuum
  • 04:21Assembly of microfluidic device
  • 04:55Cutting layers of microfluidic device
  • 06:10Bonding the microfluidic device layers
  • 08:19Coating channels with fibronectin
  • 09:08Experiment: monitoring apoptosis
  • 09:09Cell seeding
  • 11:31Application of reagents
  • 13:32Time lapse of apoptosis

Summary

Traduction automatique

Traduction automatique

We describe a protocol for the fabrication of microfluidic devices that can enable cell capture and culture. In this approach patterned microstructures such as grooves within microfluidic channels are used to create low shear stress regions within which cell can dock.

Tags

Microfluidic DeviceGroove PatternsCellular BehaviorMicrogrooved SubstrateFabricationPoly(dimethylsiloxane) (PDMS)Mouse Fibroblast CellsApoptosisOxidative StressAnnexin VPropidium IodidePhosphatidylserinePlasma MembraneReal-time ObservationInverted MicroscopeIncubation ChamberHigh-throughput Drug Screening

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/270?article_type=v

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
check_url/fr/270?article_type=v

A Gradient-generating Microfluidic Device for Cell Biology
check_url/fr/270?article_type=v

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
check_url/fr/270?article_type=v

Cell Capture Using a Microfluidic Device
check_url/fr/270?article_type=v

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
check_url/fr/270?article_type=v

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
check_url/fr/270?article_type=v

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
check_url/fr/270?article_type=v

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
check_url/fr/270?article_type=v

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
check_url/fr/270?article_type=v

Genomic MRI – a Public Resource for Studying Sequence Patterns within Genomic DNA

Read Article