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A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
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Journal JoVE Bio-ingénierie
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

DOI:
10.3791/2720-v

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07:24 min

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May 06, 2011

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1Department of Surgery,UMDNJ-Robert Wood Johnson Medical School

Chapitres

  • 00:05Titre
  • 01:14Preparing a Single Cell Suspension
  • 02:57Hanging Drop Formation
  • 04:38Representative Results of Hanging Drop Cultures, Compaction of Cellular Aggregates and Spheroid Bodies
  • 07:12Conclusion

Summary

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We describe a simple, rapid method of generating 3D tissue-like spheroids and their potential application to quantify differences in cell-cell interactions.

Tags

Hanging Drop Cell Culture3D SpheroidsCell-cell CohesionCell-substratum AdhesionMonolayer CultureRigid SubstratesIntimate ConnectionsExtracellular Matrix ComponentsChemical MilieuPhysical ForcesCellular MorphologySignalingCollagen GelsBiomaterial ScaffoldsDirect Cell-cell Adhesion ArchitectureCulture SystemsECM ProductsPhysiological ConditionsBiological Agent

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